Bile acid sulfates. II. Formation, metabolism, and excretion of lithocholic acid sulfates in the rat
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چکیده
منابع مشابه
Bile acid sulfates. II. Formation, metabolism, and excretion of lithocholic acid sulfates in the rat.
Sulfate esterification has been shown previously to be a prominent feature of lithocholate metabolism in man. These studies were undertaken to ascertain whether this metabolic pathway is also present in rats, and to investigate the physiological significance of bile acid sulfate formation. Lithocholic acid-24-(14)C was administered to bile fistula rats, and sulfated metabolites were identified ...
متن کاملBile acid sulfates. I. Synthesis of lithocholic acid sulfates and their identification in human bile.
Sulfate esters of lithocholic, glycolithocholic, and taurolithocholic acids were synthesized using sulfur trioxide in pyridine; they were purified by crystallization from methanol or ethanol as the diammonium salts, and their chemical compositions, infared spectra, and chromatographic behavior were determined. Strong alkaline hydrolysis of these sulfates, as commonly performed during quantitati...
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Milligram amounts of [3P-3H]lithocholic (3a-hydroxy5P-cholanoic) acid were administered by intravenous infusion to rats prepared with a biliary fistula. Analysis of sequential bile samples by thin-layer chromatography (TLC) demonstrated that lithocholic acid glucuronide was present in bile throughout the course of the experiments and that its secretion rate paralleled that of total isotope secr...
متن کاملMetabolism of lithocholic acid in the rat: formation of lithocholic acid 3-O-glucuronide in vivo.
Milligram amounts of [3 beta-3H]lithocholic (3 alpha-hydroxy-5 beta-cholanoic) acid were administered by intravenous infusion to rats prepared with a biliary fistula. Analysis of sequential bile samples by thin-layer chromatography (TLC) demonstrated that lithocholic acid glucuronide was present in bile throughout the course of the experiments and that its secretion rate paralleled that of tota...
متن کاملA facile hydrolysis-solvolysis procedure for conjugated bile acid sulfates.
Methods for hydrolyzing and solvolyzing conjugated bile acid sulfates were compared on reference mixtures of conjugated and unconjugated bile acid sulfates gas-liquid chromatography to assess recovery, and thin-layer chromatography and zonal scanning to define the products occurring after hydrolysis. Conventional methods in which solvolysis preceded vigorous alkaline saponification gave incompl...
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ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1971
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)39455-4